STAINING TOXOPLASMA GONDII WITH FLUORESCEINo LABELLED ANTIBODY I. Tm~ REACTION IN SMEARS OF PERITONEAL EXUDATE BY MORRIS GOLDMAN, Sc.D
نویسنده
چکیده
Toxoplasma gondii is a small organism which ordinarily is difficult to identify in tissue sections unless it occurs in clusters known as pseudocysts. A staining method specific for the organism would therefore be of value in the microscopic study of lesions suspected of being caused by this species. Since fluorescein-labelled antibody can be used to stain homologous organisms specifically (reference 1, contains extensive bibliography) a study was undertaken to see whether Toxoplasma could be stained by this method. Preliminary experiments showed that Toxoplasma in smears prepared from peritoneal fluid or organs of infected mice were readily stained with labelled antibody. Some details of this staining reaction were then studied in order to develop methods which would facilitate serologic and other investigations on toxoplasmosis. The first paper in this series describes the general methods used and the information obtained about the antigen complex which is demonstrable with labelled antibody. In the second paper, which follows immediately, the use of this staining method as the basis for a serologic test for Toxoplasma antibodies is described. Subsequent reports will deal with the problem of staining the organisms in tissue sections.
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